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Location where to get the Complete Article --> Vol 2, No 1 (2015): 44-50

ISSN- 2348-5191 (Print version); 2348-8980 (Online)

Subterranean Microhabitat Dependent Intra Versus Extracellular Enzyme Secretion Capabilities of Deinococcus radiodurans



Jayant Biswas, Arvind Neral, Yogita Rajput

Abstract

Deinococcus radiodurans is one of the most yet discovered extremophilic microbe, the isolation of which from the various habitats of Kotumsar cave is always a matter of enticement to discover its ecological economics. In the present work we studied the intra versus extracellular alkaline protease and glucose isomerase secretion capabilities of Deinococcus radiodurans; KCB21, KCB50, KCB93 isolated from three distinct subterranean niches of Kotumsar cave. The selected niches/zones were the entrance zone, transient zone and the deep inner zone from where the soil sediments were collected to isolate the bacterial strains. The results revealed high extracellular alkaline protease activity from the Deinococcus radiodurans strain which was isolated from the deeper zones of the cave, whereas no such phenomenon was revealed for glucose isomerase. The possible reason for the obtained results has been discussed.


References

  • Biswas, J (1991): Metabolic efficiency and regulation of body weight: a comparison between life in hypogean and epigean ecosystems. Int. J. Speleol., 20 (1), 15-22.
  • Biswas, J (1992a): Kotumsar cave ecosystem: An interaction between geophysical, chemical, and biological characteristics. NSS BULL. 54(1): 7-10.
  • Biswas, J (1992b): Influence of epigean environmental stress on a subterranean cave ecosystem: Kotumsar. Biome. 5(1): 39-43.
  • Biswas, J. (2010): Kotumsar Cave biodiversity: a review of cavernicoles and their troglobiotic traits. Biodivers. Conserv., 19(1): 275-289.
  • Biswas, J. & Dey, M. (2014): Troglomorphy: habitat dependent morphological divergence in two forms of a fish from Kotumsar Cave of Kanger Valley National Park, India. International Colloquium on Endocrinology and Physiology. (DOI: 10.13140/2.1.2719.8244).
  • Camassa, M. (1997): Biological observations in an artificial cave of Ginosa (Southern Italy): Role of the foval in cave ecosystems. Thalassia Salentina, 23: 189-191.
  • Duncan, D.B. (1955): Multiple range and multiple f tests. Biometrics, 11: 1-42.
  • Daly, M.J. & Minton, K.W. (1995): Resistance to radiation. Science, 270 (5240): 1318.
  • DeWeerdt, S.E. (2002): The World's Toughest Bacterium: Deinococcus radiodurans may be a tool for cleaning up toxic waste and more. Genome News Network. Available at: www.genomenewsnetwork.org/articles/07_02/deinococcus.shtml
  • Dische, Z. & Borenfreund, E. (1951): A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem., 192: 583-587.
  • Leisola, M., Jokela, J., Pastinen, O., Turunen, O. & Schoemaker, H. (2002): Industrial use of enzymes in Encyclopedia of Life Support Systems (EOLSS), O. O. P. Hänninen and M. Atalay, Eds., pp. 125, EOLSS, Oxford, UK, 2002.
  • Makarova, K.S., Aravind, L., Wolf, Y.I., Tatusov, R.L., Minton, K.W., Koonin, E.V. & Daly, M.J. (2001): Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol Rev., 65(1):44-79.
  • Nakagawa, Y. (1970): Alkaline proteases from aspergillus. Method. Enzymol. 19:581-591.
  • Rajput, Y., Rai, V. & Biswas, J. (2012): Screening of Bacterial Isolates from Various Microhabitat Sediments of Kotumsar Cave: A Cogitation on Their Respective Benefits and Expected Threats for Complete Biosphere and Tourists. Research J. Environ. Toxicol., 6 (1): 13-24.
  • Slade, D & Radman, M (2011) : Oxidative stress resistence in Deinocossus radiodurans. Microbiol. Mol. Biol. Rev., 75(1): 133-139.

  • DOI:10.21276/ambi.2015.02.1.ra06


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    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
    Published by: National Cave Research and Protection Organization, India

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